Rat tail tendon collagens protect cardiomyocytes against oxidative damage

doi:10.3969/j.issn.2095-4344.2014.52.018

Abstract

Abstract:

BACKGROUND: Although collagen has been approved to have antioxidant effect, there is no relevant research at the cell level. Previous experimental studies have focused on the effect of rat tail tendon collagens to promote cell adhesion and scaffold construction.

OBJECTIVE: To investigate the protective role of rat tail tendon collagen in oxidative stress of cardiomyocytes induced by hydrogen peroxide.

METHODS: Primary neonatal rat cardiomyocytes were seeded in culture dish with or without rat tail tendon collagen randomly, then both treated with various concentration of H₂O₂ (0, 10, 100 µmol/L) for 24 hours. Then, the morphology of cardiomyocytes was observed by inverted fluorescence microscope, the viability of cardiomyocytes wasmeasured by MTT assay, TUNEL was used to detect the apoptosis of cardiomyocytes, the percentage of apoptotic cells was determined by flow cytometry, superoxide dismutase activity was detected by xanthine oxidase method, glucosinolates barbitone colorimetry was used to detect the contents of malonaldehyde and western blot assay was used to detect the expression of Bax and Bcl-2.

RESULTS AND CONCLUSION: With the increasing of the concentration of H₂O_2, cardiomyocytes cultured with or without rat tail tendon collagen were observed severer in apoptotic state; the cell viability and superoxide dismutase activity were significantly reduced; the percentage of apoptotic cells and content of malonaldehyde were significantly increased; the Bcl-2/Bax ratio was reduced in dose-dependent manner. In the same concentration of H₂O₂, compared with the cells cultured without rat tail tendon collagen, the apoptotic state of cells cultured with rat tail tendon collagen was significantly relieved, the viability of cardiomyocytes, superoxide dismutase activity and Bcl-2/Bax ratio were higher, and the apoptosis rate and malonaldehyde content were reduced. These findings indicate that rat tail tendon collagen can protect cardiomyocytes against H₂O₂ induced oxidative stress injury through improvement of superoxide dismutase activity and Bcl-2 expression and reduction of malonaldehyde content.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: collagen, myocytes, cardiac, hydrogen peroxide

CLC Number:

R318

Bao Xin-hui, Li Xiao-mei, Tao Jing, Yang Yi-ning, Ma Yi-tong, Chen Bang-dang. Rat tail tendon collagens protect cardiomyocytes against oxidative damage [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(52): 8463-8469.

Figures/Tables 2

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